cd9 marker proteins  (Brickell Biotech)

Journal: Stem Cell Research & Therapy

Article Title: Protective effects of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on radiation-induced epididymal cells damage via USP44/RBM14 axis

doi: 10.1186/s13287-025-04782-9

Figure Lengend Snippet: Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical exosomal markers CD9, CD63, and CD81, confirming vesicle identity

Article Snippet: Exosomal protein markers CD9 (Proteintech, #20,597–1-AP, 1:2000), CD81 (Proteintech, #27,855–1-AP, 1:2000) and CD63 (Proteintech, #67,605–1-Ig, 1:5000) were detected by Western blotting.

Techniques: Flow Cytometry, Expressing, Staining, Transmission Assay, Electron Microscopy, Concentration Assay, Western Blot

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Gingipains disrupt bone homeostasis via dual regulation of osteogenesis and osteoclastogenesis through exosomal miR-146a-5p/TRAF6 signaling

doi: 10.3389/fcimb.2025.1614126

Figure Lengend Snippet: miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of exosomal miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.

Article Snippet: Exosomal marker proteins CD 9 (proteintech, 20597-1-AP, 1:4000) and CD 81 (proteintech, 27855-1- AP, 1:2000) were examined by western blot.

Techniques: In Vitro, Quantitative Proteomics, Microarray, Expressing, Transfection, Control, Software, Staining

Journal: Biological Research

Article Title: Dorsal root ganglion-derived exosomes deteriorate neuropathic pain by activating microglia via the microRNA-16-5p/HECTD1/HSP90 axis

doi: 10.1186/s40659-024-00513-1

Figure Lengend Snippet: DRG-Exo are involved in NP in SNL-induced mice. a Flow chart of mouse SNL modeling and DRG-Exo injection. Assessment of mechanical allodynia response in SNL mice using 0.07 g ( b ) or 0.4 g ( c ) von Frey filaments at different time points before and after SNL surgery, respectively. d The responses of SNL mice to thermal hyperalgesia were evaluated at different time points before and after SNL surgery. e The responses of SNL mice to cold hyperalgesia were assessed at different time points before and after SNL surgery, respectively. f Inflammatory infiltration in the spinal cord (L4) of SNL mice was observed using HE staining. g The morphology of DRG-Exo was observed using TEM. h The diameter of DRG-Exo was analyzed using NTA. i Surface markers CD9, CD63, CD81 expression and contaminant proteins Calnexin and GM130 in DRG-Exo using western blot. Assessment of mechanical allodynia response in PBS- or DRG-Exo-treated mice using 0.07 g ( j ) or 0.4 g ( k ) von Frey filaments at different time points before and after SNL surgery, respectively. l The responses of PBS- or DRG-Exo-treated mice to thermal hyperalgesia were evaluated at different time points before and after SNL surgery. m The responses of PBS- or DRG-Exo-treated mice to cold hyperalgesia were assessed at different time points before and after SNL surgery, respectively. n Inflammatory infiltration in the spinal cord (L4) of PBS- or DRG-Exo-treated mice observed using HE staining (arrows represent immune cells and the circle represents the enrichment of immune cells). Values are expressed as mean ± SD. ** p < 0.01 vs the sham group and # p < 0.05, ## p < 0.01 vs the SNL + PBS group by two-way ANOVA

Article Snippet: Total exosomal protein was measured by the bicinchoninic acid (BCA) protein concentration method, and exosomal surface marker proteins CD9 (1:1000, ab307085, Abcam, Cambridge, UK), CD63 (1:1000, ab216130, Abcam), CD81 (1:1000, ab232390, Abcam), and the contaminants calnexin (1:5000, PA5-34754, Thermo Fisher) and GM130 (1:1000, PA5-95727, Thermo Fisher) in 200 μL solution containing Exo were detected using western blot.

Techniques: Injection, Staining, Expressing, Western Blot

Journal: Biological Research

Article Title: Dorsal root ganglion-derived exosomes deteriorate neuropathic pain by activating microglia via the microRNA-16-5p/HECTD1/HSP90 axis

doi: 10.1186/s40659-024-00513-1

Figure Lengend Snippet: The scheme illustrates the mechanism of DRG-Exo in NP (by Figdraw, https://www.figdraw.com/static/index.html ). Exosomal delivery of miR-16-5p by DRG neurons interacts with HECTD1 to regulate ubiquitination levels of HSP90, thereby promoting microglial activation in NP

Article Snippet: Total exosomal protein was measured by the bicinchoninic acid (BCA) protein concentration method, and exosomal surface marker proteins CD9 (1:1000, ab307085, Abcam, Cambridge, UK), CD63 (1:1000, ab216130, Abcam), CD81 (1:1000, ab232390, Abcam), and the contaminants calnexin (1:5000, PA5-34754, Thermo Fisher) and GM130 (1:1000, PA5-95727, Thermo Fisher) in 200 μL solution containing Exo were detected using western blot.

Techniques: Activation Assay